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clock  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc clock
    Clock, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+clock/CLOCK+Rabbit+mAb/10__1172_slash_jci199056-181-17-18
    Average 95 stars, based on 78 article reviews
    clock - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Western Blot:

    Article Title: BMAL1 and CLOCK proteins in regulating UVB-induced apoptosis and DNA damage responses in human keratinocytes
    Article Snippet: Proteins were separated by SDS-PAGE and transferred onto the PVDF membrane (Millipore/Fisher Scientific, Pittsburg, PA), Darmstadt, Germany). .. Antibodies for immunoblotting from Cell Signaling Technology (Dancers, MA) would include rabbit anti-Bmal1 (#14020), rabbit anti-CLOCK (#5157), rabbit anti-phospho-p53 (Ser15) [#9284], rabbit anti-p21 Waf1/Cip1 (#2947), rabbit anti-Histone H2AX (#2595), rabbit anti-cleaved PARP (#9541), and rabbit anti-phospho-CHK1 (Ser 345) (#2348). .. Other antibodies include mouse anti-p53 (sc-126; Santa Cruz Biotechnology Inc., Santa Cruz, CA), rabbit anti-Keratin 10 (Poly19054; Biolegend, San Diego, CA), mouse anti-phospho-Histone H2AX (Ser139) (05–636; Millipore/Fisher Scientific), and mouse anti-α-tubulin (T9026; Sigma-Aldrich, St. Louis, MO).

    Article Title: Neurodegeneration-associated FUS is a novel regulator of circadian gene expression
    Article Snippet: .. The primary antibodies used are: rabbit anti-PSF (Sigma, PLA0181; WB: 1:2,000, IP: 1:100; ChIP: 1:100), mouse anti-FUS (Santa Cruz, sc-4H11; WB: 1:1,000), mouse anti-DYKDDDDK-Tag (FLAG) (Abmart, M2008; WB:1:5,000), mouse anti-DYKDDDDK-Tag conjugated protein A/G beads (Abmart, M200018; IP and ChIP: 35uL beads per each assay), mouse anti-PSF antibody (Sigma, P2860; WB: 1:1,000), rabbit anti-CLOCK (Cell Signaling Technology, D45B10; WB:1:3,000), rabbit anti-BMAL1 (CST, D2L7G; WB: 1:3,000), rabbit anti-PER2 (Abcam, ab179813; WB: 1:1,000), mouse anti-ACTIN (Abmart, M20010; WB: 1:5,000), rabbit anti HDAC1 (Abcam, ab7028; ChIP: 1:150; WB: 1:2,000), mouse anti-TUBULIN (Abmart, T40103; WB: 1:5,000), mouse-anti GAPDH (Proteintech, 60004-1-Ig; WB: 1:10,000). ..

    Modification:

    Article Title: The non-classical nuclear import carrier Transportin 1 modulates circadian rhythms through its effect on PER1 nuclear localization
    Article Snippet: Image analysis were performed using ImageJ 1.44p. .. We performed a modified (unpublished) standard chromatin immunoprecipitation (ChIP) in triplicates using 1 mg of formaldehyde cross-linked protein extracts from unsynchronized U-2 OS with the following antibodies: rabbit anti-CLOCK (Abcam), rabbit anti-CLOCK (Cell Signaling) and rabbit anti-IgG (Cell Signaling). .. After the final wash of the ChIP, proteins bound to ChIP-grade agarose beads (Cell Signaling) were digested into peptides as follows.

    Chromatin Immunoprecipitation:

    Article Title: The non-classical nuclear import carrier Transportin 1 modulates circadian rhythms through its effect on PER1 nuclear localization
    Article Snippet: Image analysis were performed using ImageJ 1.44p. .. We performed a modified (unpublished) standard chromatin immunoprecipitation (ChIP) in triplicates using 1 mg of formaldehyde cross-linked protein extracts from unsynchronized U-2 OS with the following antibodies: rabbit anti-CLOCK (Abcam), rabbit anti-CLOCK (Cell Signaling) and rabbit anti-IgG (Cell Signaling). .. After the final wash of the ChIP, proteins bound to ChIP-grade agarose beads (Cell Signaling) were digested into peptides as follows.

    Article Title: Neurodegeneration-associated FUS is a novel regulator of circadian gene expression
    Article Snippet: .. The primary antibodies used are: rabbit anti-PSF (Sigma, PLA0181; WB: 1:2,000, IP: 1:100; ChIP: 1:100), mouse anti-FUS (Santa Cruz, sc-4H11; WB: 1:1,000), mouse anti-DYKDDDDK-Tag (FLAG) (Abmart, M2008; WB:1:5,000), mouse anti-DYKDDDDK-Tag conjugated protein A/G beads (Abmart, M200018; IP and ChIP: 35uL beads per each assay), mouse anti-PSF antibody (Sigma, P2860; WB: 1:1,000), rabbit anti-CLOCK (Cell Signaling Technology, D45B10; WB:1:3,000), rabbit anti-BMAL1 (CST, D2L7G; WB: 1:3,000), rabbit anti-PER2 (Abcam, ab179813; WB: 1:1,000), mouse anti-ACTIN (Abmart, M20010; WB: 1:5,000), rabbit anti HDAC1 (Abcam, ab7028; ChIP: 1:150; WB: 1:2,000), mouse anti-TUBULIN (Abmart, T40103; WB: 1:5,000), mouse-anti GAPDH (Proteintech, 60004-1-Ig; WB: 1:10,000). ..



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